withania coagulans extract induces cell apoptosis and inhibits cox-2 expression in a rat model of benign prostatic hyperplasia
نویسندگان
چکیده
background phytotherapy is a popular treatment option in cases of benign prostatic hyperplasia (bph), with many different herbal products being used for the treatment of this condition. withania coagulans (wc) is an herbal medicine that has shown anti-tumoral, anti-inflammatory, and antioxidant effects. objectives this study examined the effect of withania coagulans extract (wce) on prostatic cell apoptosis and cyclooxygenase-2 (cox-2) expression in cases of benign prostatic hyperplasia (bph) in rats. methods forty wistar rats were equally divided into five groups: control, sham, bph, bph + wce, and bph + clx (celecoxib) as a positive control group. the induction of bph was achieved via the subcutaneous injection of 3 mg/kg of testosterone propionate (tp) daily for 28 days. the animals received wce, celecoxib, or distilled water by oral gavage accompanied by the tp injection. after four weeks, the prostate glands of the rats were weighed to measure the prostatic index (pi). the ventral lobes of the prostates were dissected and processed with paraffin blocks in order to study the number of mast cells. a tunel analysis was performed to evaluate the cell apoptosis, while the expression of cox-2 was examined using immunohistochemistry. results bph was obvious in the ventral lobe of the prostate, and the administration of wce markedly decreased the pi and the number of mast cells (p < 0.001) in the bph rats. additionally, the wce treatment induced prostatic cell apoptosis when compared to the bph group. furthermore, following the wce treatment, the expression of cox-2 in the prostatic tissues was significantly decreased when compared to the bph groups. conclusions according to the results of this study, wce was effective in the treatment of bph in rats. it may therefore have beneficial effects in the treatment of patients with bph.
منابع مشابه
Withania coagulans Extract Induces Cell Apoptosis and Inhibits COX-2 Expression in a Rat Model of Benign Prostatic Hyperplasia
BACKGROUND Phytotherapy is a popular treatment option in cases of benign prostatic hyperplasia (BPH), with many different herbal products being used for the treatment of this condition. Withania coagulans (WC) is an herbal medicine that has shown anti-tumoral, anti-inflammatory, and antioxidant effects. OBJECTIVES This study examined the effect of Withania coagulans extract (WCE) on prostatic...
متن کاملAntiproliferative and Antioxidant Effects of Withania coagulans Extract on Benign Prostatic Hyperplasia in Rats
BACKGROUND Benign prostate hyperplasia (BPH) is a common urological disorder in elderly men. Phytotherapy is frequently used to alleviate the symptoms of this condition. OBJECTIVES The present study investigated the effect of Withania coagulans extract (WCE), which is known to have antioxidant, anti-inflammatory, antihyperglycemic, and anti-cancer properties, on testosterone-induced BPH in ra...
متن کاملantiproliferative and antioxidant effects of withania coagulans extract on benign prostatic hyperplasia in rats
conclusions w. coagulans inhibits the development of bph can be useful for the treatment of this condition. results the present study revealed that bph caused elevation of mda levels, suppression of tac levels, and increased pcna expression in the prostate gland. interestingly, in a dose-dependent manner, wce caused decreased mda levels and increased tac levels in the prostate gland, compared t...
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عنوان ژورنال:
nephro-urology monthlyجلد ۸، شماره ۵، صفحات ۰-۰
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